[Tyra Talk] 2024-06-22 DNA 編碼調控的水凝膠
DyNAtrix是一種新型的DNA水凝膠，它擁有自癒性、可列印性、高穩定性、與細胞和血液相容性高，且科學家可以控制其分解。相較於傳統常用的基質，以DyNAtrix培養的人類幹細胞及器官樣結構顯示出高存活率、增殖和形態發生。因此， DyNAtrix極其適用於未來生物力學、生物物理學和組織工程的實驗與研究。彭于瑄是2023Nature Nanotechnology (Impact Factor 38.3!) DyNAtrix研究的第一作者，而他將於6/22在TYRA平台分享他最先進的研究成果！
|| 講者（Speaker）:
彭于瑄（Yu-Hsuan Peng）
交大生物科技系-->大四來德國羅特林根做交換，對生物材料產生興趣，於是同時申請德國碩班-->開始在 TU Dresden 讀 Regenerative Biology and Medicine -->碩論開始做 DNA hydrogels --> PhD 跟著同一個老闆繼續發展材料。念碩班時認識了水凝膠的特性與應用，並對水凝膠產生濃厚的興趣。碩論期間，在現任老闆的指導下成功用 DNA crosslinks 做出初版的水凝膠，經過改良後，我們已經可以用來培養多種細胞與類器官，藉此來研究細胞面對不同材料特性的反應。 (把博班當成夏令營在念，平日喜歡運動與拖延。碩班期間認識了法蘭克，在他畢業後接任同學會會長，素來景仰法蘭克的為人與辦事效率，收到他的邀(脅)請(迫)我感到非常榮幸，很高興可以跟大家分享我們實驗室的研究成果。)
|| 摘要（Abstract）:
Three-dimensional cell and organoid cultures rely on the mechanical support of viscoelastic matrices. However, commonly used matrix materials lack control over key cell-instructive properties. Here we report on fully synthetic hydrogels based on DNA libraries that self-assemble with ultrahigh-molecular-weight polymers, forming a dynamic DNA-crosslinked matrix (DyNAtrix). DyNAtrix enables computationally predictable and systematic control over its viscoelasticity, thermodynamic and kinetic parameters by changing DNA sequence information. Adjustable heat activation allows homogeneous embedding of mammalian cells. Intriguingly, stress-relaxation times can be tuned over four orders of magnitude, recapitulating mechanical characteristics of living tissues. DyNAtrix is self-healing, printable, exhibits high stability, cyto- and haemocompatibility, and controllable degradation. DyNAtrix-based cultures of human mesenchymal stromal cells, pluripotent stem cells, canine kidney cysts and human trophoblast organoids show high viability, proliferation and morphogenesis. DyNAtrix thus represents a programmable and versatile precision matrix for advanced approaches to biomechanics, biophysics and tissue engineering.
|| 研究領域（Field）:
自然科學、數學、統計
|| 研究子領域（Sub-field）:
Molecular Biology, Mechanobiology, Tissue engineering, Rheology
|| 其他關鍵字（Supplementary keywords）:
supramolecular hydrogels, ECM, 3D cell culture, organoids
|| 主持人（Chair）:
|| 圖像設計（Design）:
|| 活動時間（Event Time）:
06/22/2024 07:00 AM PDT Pacific Time
06/22/2024 08:00 AM MDT Mountain Time
06/22/2024 09:00 AM CDT Central Time
06/22/2024 10:00 AM EDT Eastern Time
06/22/2024 03:00 PM BST England
06/22/2024 04:00 PM CEST Berlin
06/22/2024 10:00 PM Taiwan
關於Project Tyra更多詳情請參見
https://linktr.ee/projecttyra